abstract：:The distribution of neuronal elements immunoreactive for gamma-aminobutyric acid (GABA) and glycine in the cochlear nucleus of the mustached bat Pteronotus parnellii has been studied by means of the postembedding technique on serial semithin sections. Our goal has been to identify similarities and differences in the o...

journal_title：Cell and tissue research

authors： Kemmer M,Vater M

更新日期：1997-02-01 00:00:00

abstract：:Bone resorption by mononucleated cells was studied in the acellular bone of a teleost fish (Oreochromis niloticus) by histological and enzyme histochemical observations and by transmission electron microscopy. Bone resorbing cells (osteoclasts) were identified by their location at the sites of bone resorption, their f...

journal_title：Cell and tissue research

authors： Witten PE,Bendahmane M,Abou-Haila A

更新日期：1997-02-01 00:00:00

abstract：:Biocytin, recently introduced in neuroanatomical studies, was used as a retrograde tract tracer in combination with immunofluorescence in order to analyse the neurochemical characters of some central neuronal projections to the pars intermedia in two amphibian species, the anuran Rana esculenta and the urodele Trituru...

journal_title：Cell and tissue research

authors： Jansen K,Fabro C,Artero C,Feuilloley M,Vaudry H,Fasolo A,Franzoni MF

更新日期：1997-02-01 00:00:00

abstract：:Previously, we showed that synchronous autophagy occurred in the corpora allata during the declining phase of juvenile hormone synthesis in mated adult females of the cockroach Diploptera punctata. At the onset of this process, conspicuous whorl-like autophagosomes were often observed. Each consisted of a ribosome-con...

journal_title：Cell and tissue research

authors： Yang DM,Chiang AS

更新日期：1997-02-01 00:00:00

abstract：:Transforming growth factor beta (TGF-beta) is a major member of a cytokine family with pluripotent biological activity. In the liver, TGF-beta has pathophysiological significance with respect to hepatic fibrogenesis, regulation of liver cell growth, tumor development, and induction of hepatocellular apoptosis. We show...

journal_title：Cell and tissue research

authors： Gressner AM,Wulbrand U

更新日期：1997-01-01 00:00:00

abstract：:We have previously shown that mice deficient in the gene for the myelin-associated glycoprotein (MAG) develop normal myelin in the peripheral nerves, but show axon and myelin degeneration at eight months of age, suggesting that MAG is involved in the maintenance of axon-Schwann cell integrity. The search for molecules...

journal_title：Cell and tissue research

authors： Carenini S,Montag D,Cremer H,Schachner M,Martini R

更新日期：1997-01-01 00:00:00

abstract：:The oxyntic mucosa of the mammalian stomach is rich in endocrine cells, such as ECL cells, A-like cells, somatostatin cells, D1/P cells and, in some species, enterochromaffin cells. The various endocrine cell types can be distinguished on the basis of their characteristic cytoplasmic granules and vesicles. The ECL cel...

journal_title：Cell and tissue research

authors： Andersson K,Zhao CM,Chen D,Sundler F,Hâkanson R

更新日期：1996-12-01 00:00:00

abstract：:Hepatic stellate cells become activated and aquire contractility on being cultured. In order to characterize the morphology of contracted and relaxed stellate cells, we performed light- and electron-microscopic analyses of cultured stellate cells on collagen gels. Incubation of stellate cells with medium alone, 10 nM ...

journal_title：Cell and tissue research

authors： Kawada N,Harada K,Ikeda K,Kaneda K

更新日期：1996-12-01 00:00:00

abstract：:During normal development of many vertebrate species, substantial numbers of neurons in the central and peripheral nervous system undergo naturally occurring (or programmed) cell death. For example, approximately 50% of spinal motoneurons degenerate and die at a time when these cells are establishing synaptic connecti...

journal_title：Cell and tissue research

authors： Houenou LJ,Oppenheim RW,Li L,Lo AC,Prevette D

更新日期：1996-11-01 00:00:00

abstract：:The subcommissural organ secretes N-linked complex-type glycoproteins into the cerebrospinal fluid. These glycoproteins condense to form Reissner's fiber (RF), which extends along the fourth ventricle and central canal of the spinal cord. A set of three monoclonal antibodies (Mabs 3E6, 3B1, and 2A5) has been obtained ...

journal_title：Cell and tissue research

authors： Pérez J,Garrido O,Cifuentes M,Alonso FJ,Estivill-Torrús G,Eller G,Nualart F,López-Avalos MD,Fernández-Llebrez P,Rodríguez EM

更新日期：1996-10-01 00:00:00

abstract：:The effect of hypothyroidism, induced by 4-propyl-2-thiouracil, on muscle satellite cells in vivo and in vitro, and on postnatal muscle fiber development in the soleus muscle of rats during the first 40 days of postnatal life was analyzed. The proliferative activity of satellite cells was determined by means of bromod...

journal_title：Cell and tissue research

authors： Jacobs SC,Bär PR,Bootsma AL

更新日期：1996-10-01 00:00:00

abstract：:The circadian timing system is a neural network consisting of the hypothalamic suprachiasmatic nucleus, aided by the retina, other hypothalamic nuclei, autonomic regions of the spinal cord, sympathetic ganglia and the pineal gland. Extensive studies conducted over the last two decades have unravelled the principal ite...

journal_title：Cell and tissue research

authors： Reuss S

更新日期：1996-09-01 00:00:00

abstract：:Although the pharmacological properties and distribution of inositol 1,4,5-trisphosphate (IP3)-sensitive calcium stores vary considerably among tissues, studies of its localization have been devoted mostly to the central nervous system. In this report we have analyzed the localization of IP3 receptors in diverse non-n...

journal_title：Cell and tissue research

authors： Nakanishi S,Fujii A,Nakade S,Mikoshiba K

更新日期：1996-08-01 00:00:00

abstract：:Rainbow trout (Oncorhynchus mykiss) were exposed to ion-poor (soft) water to test the hypothesis that naturally induced proliferation of branchial chloride cells causes a thickening of the blood-to-water diffusion barrier. This was achieved by using a combination of scanning and transmission electron-microscopic techn...

journal_title：Cell and tissue research

authors： Greco AM,Fenwick JC,Perry SF

更新日期：1996-07-01 00:00:00

abstract：:The ciliary axoneme in photoreceptors from the retina of Xenopus laevis was examined by immunofluorescent staining of tubulin throughout the light/dark cycle. The immunofluorescent axoneme extended along only part of the length of rod outer segments but the entire length of cone outer segments. Both the cone axoneme a...

journal_title：Cell and tissue research

authors： Eckmiller MS

更新日期：1996-07-01 00:00:00

abstract：:An antiserum against the octapeptide Pea-CAH-I, a member of the adipokinetic hormone/red pigment-concentrating hormone family, has been produced for immunocytochemical staining in insects and various other invertebrate species. The anti-Pea-CAH-I serum stains the glandular corpora cardiaca cells of those insect specie...

journal_title：Cell and tissue research

authors： Eckert M,Gabriel J,Birkenbeil H,Greiner G,Rapus J,Gäde G

更新日期：1996-06-01 00:00:00

abstract：:Activities of the H+, K(+)-ATPase and the Na+, K+, ATPase have been localized in the morphologically heterogeneous oxyntic cell lineage of adult bovine abomasal mucosa, by means of K(+)-dependent paranitrophenylphosphatase (K(+)-pNPPase) histochemistry. At the light- and electron-microscopic level, only members of the...

journal_title：Cell and tissue research

authors： Kressin M

更新日期：1996-05-01 00:00:00

abstract：:Expression of heat-shock protein 47 in intact and fibrotic liver and in hepatic constituent cells was investigated in mice. Immunohistochemical study of intact liver and Western blot analysis of the protein from isolated liver cells revealed that stellate cells and smooth muscle cells of interlobular vessels, but not ...

journal_title：Cell and tissue research

authors： Kawada N,Kuroki T,Kobayashi K,Inoue M,Nakatani K,Kaneda K,Nagata K

更新日期：1996-05-01 00:00:00

abstract：:The high affinity IgE receptor on mast cells and basophils is composed of 3 subunits, alpha, beta and gamma. A polyclonal antibody was raised in rabbits to a 13 amino acid synthetic peptide corresponding to the amino terminal portion of the gamma subunit. The antiserum was screened using Western blots of solubilized R...

journal_title：Cell and tissue research

authors： Jamur MC,Oliver C

更新日期：1996-04-01 00:00:00

abstract：:Bone morphogenetic proteins (BMPs) induce cartilage and bone formation at both bony and non-bony sites. We examined the possibility whether BMP-2 induces differentiation of osteoblast progenitors into chondroblast lineage cells using organ culture and cell culture prepared from the calvaria of newborn mouse. BMP-2 sti...

journal_title：Cell and tissue research

authors： Komaki M,Katagiri T,Suda T

更新日期：1996-04-01 00:00:00

abstract：:The adrenal gland of mammals contains high concentrations of peripheral-type benzodiazepine receptors (PBR) and diazepam-binding inhibitor (DBI), a polypeptide which acts as an endogenous ligand for PBR. The aim of the present study was to investigate the localization of DBI and PBR in the adrenal gland of the frog Ra...

journal_title：Cell and tissue research

authors： Lesouhaitier O,Feuilloley M,Lihrmann I,Ugo I,Fasolo A,Tonon MC,Vaudry H

更新日期：1996-03-01 00:00:00

abstract：:Antibodies raised against the cystic fibrosis transmembrane regulator protein (CFTR) were used to localize CFTR in intestinal tissues of piglets and mice. Positive staining for CFTR was detected in goblet cells of both species. A second population of epithelial cells of unknown phenotype was also labeled by anti-CFTR ...

journal_title：Cell and tissue research

authors： Hayden UL,Carey HV

更新日期：1996-02-01 00:00:00

abstract：:The organization of the neuropeptide Y (NPY)-immunoreactive system in the forebrain, pineal organ and retina of a biweekly spawning fish (Fundulus heteroclitus) was investigated. Immunoreactivity was encountered in neurons of the nucleus olfactoretinalis, in the large population of neurons in the floor of the telencep...

journal_title：Cell and tissue research

authors： Subhedar N,Cerdá J,Wallace RA

更新日期：1996-02-01 00:00:00

abstract：:The spatial and temporal distribution of three peptides, DSK I, DSK II, and DSK 0, encoded by the Drosophila melanogaster drosulfakinin (Dsk) gene, have been examined in the central nervous system. DSK I and DSK II have a -RFamide C-terminus and are structurally similar to sulfakinin peptides; in contrast, DSK 0 conta...

journal_title：Cell and tissue research

authors： Nichols R,Lim IA

更新日期：1996-01-01 00:00:00

abstract：:Horseradish peroxidase (HRP) was injected into the left dorsal column nuclei of adult cats. Large dorsal funiculi axons of the C3, C5, C8 and L7 segments were searched for HRP-activity after 12, 24, 36 and 48h using light and electron microscopy. Accumulations of intra-axonal HRP-positive bodies occurred at nodes of R...

journal_title：Cell and tissue research

authors： Fabricius C,Berthold CH,Gatzinsky KP

更新日期：1996-01-01 00:00:00

abstract：:Bovine granulosa cells from 3-7 mm follicles were cultured without anchorage in soft agar/methylcellulose solution for 14 days, with or without 50 ng/ml basic fibroblast growth factor. The granulosa cells divided to form colonies of cells. These were analysed by light and electron microscopy, immunohistochemistry and ...

journal_title：Cell and tissue research

authors： Rodgers HF,Lavranos TC,Vella CA,Rodgers RJ

更新日期：1995-12-01 00:00:00

abstract：:Boar spermadhesin (AWN) is a 14-kDa multifunctional protein, attached to the surface of the spermatozoa and involved in sperm capacitation and zona pellucida binding. The cellular origin of AWN was previously unknown. Moreover, the region of the male genital tract in which AWN becomes attached to the surface of sperma...

journal_title：Cell and tissue research

authors： Sinowatz F,Amselgruber W,Töpfer-Petersen E,Calvete JJ,Sanz L,Plendl J

更新日期：1995-10-01 00:00:00

abstract：:The development of the canine proximal colon from the completion of organogenesis through 43 days after birth was studied using light microscopy, immunofluorescence and electron microscopy. During this period the tunica muscularis increased in thickness from 42+/-6 microm in animals midway through the gestation period...

journal_title：Cell and tissue research

authors： Ward SM,Torihashi S

更新日期：1995-10-01 00:00:00

abstract：:alpha-Smooth muscle (alpha SM) actin of endothermic vertebrates is selectively recognized by the monoclonal antibody anti-alpha SM-1. Immunoreactivity to this antibody has been shown to be localized in the NH2-terminal sequence Ac-EEED (Chaponnier et al. 1994). Among terrestrial ectothermic vertebrates, two amphibian ...

journal_title：Cell and tissue research

authors： Di Rosa I,Panara F,Fagotti A,Simoncelli F,Chaponnier C,Gabbiani G,Pascolini R

更新日期：1995-09-01 00:00:00

abstract：:The spermatogonial stem cell line in prepubertal and adult bovine testis was studied by electron microscopy and protein gene product 9.5 immunohistochemistry. Three successive spermatogonia precursor cell configurations were observed. Small basal stem cells were found to possess a spherical shape and nuclei with two t...

journal_title：Cell and tissue research

authors： Wrobel KH,Bickel D,Kujat R,Schimmel M

更新日期：1995-08-01 00:00:00

abstract：:Maternal immunoglobulin-G (IgG) is known to be transported across the placental syncytiotrophoblast during the period when the human fetus is incapable of manufacturing these defensive molecules. In this study we investigated the possible role of the amniochorion, that surrounds the amniotic cavity in which the fetus ...

journal_title：Cell and tissue research

authors： Bright NA,Ockleford CD

更新日期：1995-08-01 00:00:00

abstract：:Although the cerebrospinal fluid-contacting neurons of the avian paraventricular organ exhibit considerable amounts of catecholamines, they show no tyrosine hydroxylase immunoreactivity. In the quail embryo, the development of these neurons has been studied using the paraformaldeyde-glutaraldeyde method for the fluore...

journal_title：Cell and tissue research

authors： Guglielmone R

更新日期：1995-07-01 00:00:00

abstract：:Using isoenzyme-specific antisera, we have studied the distribution of protein kinase C isoforms in fetal mouse organs at the developmental age of 17 days. Two different sets of antibodies, produced by different manufacturers, were employed in this study. The specificity of the antisera was tested by immunoblotting ex...

journal_title：Cell and tissue research

authors： Bareggi R,Grill V,Zweyer M,Narducci P,Martelli AM

更新日期：1995-06-01 00:00:00

abstract：:Double-labeling immunofluorescent histochemistry demonstrates that calretinin, a calcium-binding protein, coexists with calcitonin gene-related peptide, vasoactive intestinal peptide, and substance P in the fibers innervating the lamina propria of the rat intestinal villi. An acetylcholinesterase histochemical stain r...

journal_title：Cell and tissue research

authors： Isaacs KR,Winsky L,Strauss KI,Jacobowitz DM

更新日期：1995-06-01 00:00:00

abstract：:The inositol 1,4,5-trisphosphate receptor (IP3R) is an intracellular Ca2+ release channel responsible for mobilizing stored Ca2+. Three different receptor types have been molecularly cloned, and their genes have been classified into a family. The gene for the type 1 receptor (IP3R1) is predominantly expressed in cereb...

journal_title：Cell and tissue research

authors： Fujino I,Yamada N,Miyawaki A,Hasegawa M,Furuichi T,Mikoshiba K

更新日期：1995-05-01 00:00:00

abstract：:Dorsal unpaired median (DUM) neurones in the abdominal ganglia of the locust were impaled with microelectrodes and some were injected intracellularly with horseradish peroxidase so that their synapses could be identified in the electron microscope. Simultaneous recordings from DUM neurones in different abdominal gangl...

journal_title：Cell and tissue research

authors： Pflüger HJ,Watson AH

更新日期：1995-05-01 00:00:00

abstract：:A synthetic 17-mer peptide corresponding to an unique sequence in the amino-terminal region of human creatine kinase B was used to raise a new and highly B-subunit-specific monoclonal antibody, CK-BYK/21E10. We show here that the monoclonal antibody is suitable for immunohistochemistry of unfixed frozen sections as we...

journal_title：Cell and tissue research

authors： Sistermans EA,de Kok YJ,Peters W,Ginsel LA,Jap PH,Wieringa B

更新日期：1995-05-01 00:00:00

abstract：:The median eminence/pituitary stalk represents the final common pathway for fibers from neurons that project to the pituitary gland. We have used the lipophilic fluorescent tracer 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) to determine the location of neurons projecting to the median emin...

journal_title：Cell and tissue research

authors： Knapp R,Silver R

更新日期：1995-04-01 00:00:00

abstract：:Peptidyl-glycine alpha-amidating monooxygenase (PAM; EC 1.14.17.3) is an enzyme that catalyzes conversion of glycine-extended peptides to alpha-amidated bioactive peptides. Two peptides that are processed at their carboxyl-termini by this enzyme are neuropeptide Y and anglerfish peptide Y, both of which possess a C-te...

journal_title：Cell and tissue research

authors： McDonald JK,Klein K,Noe BD

更新日期：1995-04-01 00:00:00

abstract：:The medial preoptic nucleus is a sexually dimorphic structure whose cytoarchitecture, afferent and efferent connections, and functions have been previously described. No detailed ultrastructural study has, however, been performed to date. Here we describe the ultrastructural organization of this important preoptic str...

journal_title：Cell and tissue research

authors： Panzica GC,Spigolon S,Castagna C

更新日期：1995-03-01 00:00:00